Hugo Miguel Baptista Carreira dos Santos

Investigador Auxiliar
Secção de Bioquímica e Biofísica
(+351) 212948300

I graduated in Applied Chemistry from the NOVA University Lisbon and completed a PhD in Biochemistry from the same university in 2010. During my PhD, I spent six months at the Turku Centre for Biotechnology in Finland, working with state-of-the-art mass spectrometry (MS) for biomedical research (3 publ, Cit. =33, as of June 2022). Then I took up a post-doc at the University of Vigo, followed by a move to the Institute for Biomedicine and Biotechnology (Barcelona, Spain, 2011–2012) to advance biomedical applications of MS and Imaging-MS (13 publ, Cit.=140). In 2013 I moved to FCT-NOVA (Portugal), where I developed interdisciplinary research using nano-proteomics, supramolecular separation, MS, and computational methods for early detection of pathologies and reliable disease screening (33 publ, Cit.=490, 4 Master students). 
In 2016 I was awarded the prestigious FCT Investigator Starting Grant, where I played a leading role in implementing label-free MS-based quantitative proteomics methods to investigate renal cell carcinoma, bladder cancer, neurodegeneration, and other ageing-related diseases (38 publ, C1=300, Co-supervision of 4 PhD students, 5 Master students). Also, in 2016, I secured private competitive funding from PROTEOMASS Scientific Society, totalling 650 k€, to set up a state-of-the-art mass spectrometry facility (https://www.bioscopegroup.org/facility/). This facility is equipped with two high-resolution nanoLC-ESI-qQ TOF instruments and bioinformatics platforms that are used to identify the molecules involved in complex biological processes, characterise their structure, and monitor how their abundance or structure may change during these processes to gain insights into the underlying molecular mechanisms.

Áreas de Investigação

  • Oncoproteomics
  • Immunopeptidomics
  • Non-canonical Antigens
  • Clinical Biochemistry

Interesses Científicos

  • Mass spectrometry-based Proteomics
  • Label-free Protein Quantification
  • Clinical Biomarkers Discovery and Development

Publicações Representativas

Carvalho, L.B., Capelo, J.L., Lodeiro, C. et al. Pathway-guided monitoring of the disease course in bladder cancer with longitudinal urine proteomics. Commun Med 3, 8 (2023). https://doi.org/10.1038/s43856-023-00238-4

Ribeiro, M., Sousa, M., Borges, V. et al.  Bioinformatics study of expression from genomes of epidemiologically related MRSA CC398 isolates from human and wild animal samples. J. Proteomics 268, 104714 (2021). https://doi.org/10.1016/j.jprot.2022.104714

Jorge, S., Capelo, J.L., LaFramboise, W. et al. Absolute quantitative proteomics using the total protein approach to identify novel clinical immunohistochemical markers in renal neoplasms. BMC Med 19, 196 (2021). https://doi.org/10.1186/s12916-021-02071-9

Carvalho, L., Capelo-Martinez, J.L., Lodeiro, C. et al. Ultrasonic-based filter aided sample preparation as the general method to sample preparation in proteomics. Anal. Chem. 2020, 92, 13, 9164–9171. https://doi.org/10.1021/acs.analchem.0c01470

Susana, J., Capelo, J.L., LaFramboise, W. et al. Development of a Robust Ultrasonic-based sample treatmentto unravel the proteome of OCT-embedded solid tumor biopsies. J. Proteome Res. 2019, 18, 7, 2979-2986. https://doi.org/10.1021/acs.jproteome.9b00248



Laboratory for Biological Mass Spectrometry