Maria Gabriela Machado de Almeida

Investigador Auxiliar
Secção de Bioquímica e Biofísica

Gabinete: 436-A
Laboratório: 407

Telefone Departamental: (+351) 212948300 Extensão: 10957
Fax: (+351) 212948550
Email: gabriela.almeida@fct.unl.pt

Interesses Científicos

- Purificação e caracterização de proteínas isoladas de bactérias desnitrificantes e redutoras de sulfato.

- Electroquímica de Proteínas.
- Modificação de superfícies electródicas (nanotubos de carbono, monocamadas auto-montadas, nanopartículas de ouro).
- Biossensores enzimáticos.

- Proteómica e Electroforese 2D.

Publicações Representativas

M.G. Almeida, S. Macieira, L.L. Gonçalves, R. Huber, C.A. Cunha, M.J. Romão,C. Costa, J. Lampreia, J.J.G. Moura, I. Moura (2003) “The Isolation and characterization of Cytochrome c Nitrite Reductase Subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774. Re-evaluation of the spectroscopic data and redox properties”, Eur. J. Biochem. 270, 3904-3915.

S. da Silva, S. Cosnier, M.G. Almeida, J.J.G. Moura (2004) “An Efficient Poly(pyrrole)-Nitrite Reductase Biosensor for the Mediated Detection of Nitrite”, Electrochem. Comm. 6, 404-408.

M.G. Almeida, C.M. Silveira, B. Guigliarelli, P. Bertrand, JJ.G. Moura, I. Moura, C. Léger (2007) “A Needle in a Haystack: the Active Site of the Membrane-bound Complex Cytochrome c Nitrite Reductase”, FEBS Lett. 581(2), 284-288.

M.G. Almeida, C.M. Silveira, J.J.G. Moura (2007) “Biosensing Nitrite Using the System Nitrite Redutase/Nafion/Methyl Viologen - A Voltammetric Study”, Biosens. Bioelectron. 22(11), 2485-2492.

H. Chen, C. Mousty, S. Cosnier, C. Silveira, J.J.G. Moura, M.G. Almeida (2007) “Highly Sensitive ccNiR Biosensor Electrical-Wired by [ZnCrAQS2] for Nitrite Determination”, Electrochem. Comm. 9, 2240-2245.

C.M. Silveira, S.P. Gomes, A.N. Araújo, C.M.C.M. Couto, M.C.B.S.M. Montenegro, R. Silva, A.S. Viana, S. Todorovic, J.J.G. Moura, M.G. Almeida (2010) “An Efficient Mediatorless Biosensor for Nitrite Determination”, Biosens. Bioelectron., 25(9) 2026-2032.

C. Silveira, J. Baur, S. Cosnier, J.J.G. Moura, M. Holzinger, S. Cosnier, M.G. Almeida (2010) "Enhanced direct electron transfer of a multihemic nitrite reductase on SWCNT modified electrodes", Electroanalysis, 22 (24), 2973–2978.

M.G. Almeida, A.S. Serra, C. Silveira, J.J.G. Moura (2010) ”Nitrite Biosensing Via Selective Enzymes – A Long But Promising Route”, Sensors, 10(12) 11530-11555; doi:10.3390/s101211530.

A.S. Serra, S. Jorge, C. Silveira, J.J.G. Moura, E. Jubete, E. Ochoteco, M.G. Almeida (2011) ”Cooperative use of Cytochrome cd1 Nitrite Reductase and its Redox Partner Cytochrome c552 to Improve the Selectivity of Nitrite Biosensing” Anal. Chim. Acta, 693 (1-2), 41-46.