Module I - Theory
Synthesis and characterization of nanoparticles
Nanoparticle-based sample fractionation
Electrophoresis
Protein identification by Mass Spectrometry
Nanoprofiling
Module II - Hand-on
Experimental design
Sample collection storage and preservation
Sample clean-up
Protein quantification
Nanoparticle synthesis characterization and applications:
Protein fractionation using gold-nanoparticles (serum samples)
Characterization of GNPs-protein complexes (UV-Vis, SDS-PAGE)
In-gel and In-solution protein digestion (GNPs-protein complexes)
Protein identification using MALDI-TOF/TOF MS
Data analysis