Maria Gabriela Machado de Almeida

Associate researcher
Secção de Bioquímica e Biofísica
(+351) 212948300
(+351) 212948550

Research Interests

- Purification and characterization of proteins isolated from denitrifing and sulphate reducing bacteria.

- Electrochemistry of proteins.
- Modification of electrode surfaces (carbon nanotubes, self-assembled monolayers, gold nanoparticles).
- Enzyme based electrochemical biosensors.

- Proteomics and 2D Electrophoresis.

Main Publications

M.G. Almeida, S. Macieira, L.L. Gonçalves, R. Huber, C.A. Cunha, M.J. Romão,C. Costa, J. Lampreia, J.J.G. Moura, I. Moura (2003) “The Isolation and characterization of Cytochrome c Nitrite Reductase Subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774. Re-evaluation of the spectroscopic data and redox properties”, Eur. J. Biochem. 270, 3904-3915.

S. da Silva, S. Cosnier, M.G. Almeida, J.J.G. Moura (2004) “An Efficient Poly(pyrrole)-Nitrite Reductase Biosensor for the Mediated Detection of Nitrite”, Electrochem. Comm. 6, 404-408.

M.G. Almeida, C.M. Silveira, B. Guigliarelli, P. Bertrand, JJ.G. Moura, I. Moura, C. Léger (2007) “A Needle in a Haystack: the Active Site of the Membrane-bound Complex Cytochrome c Nitrite Reductase”, FEBS Lett. 581(2), 284-288.

M.G. Almeida, C.M. Silveira, J.J.G. Moura (2007) “Biosensing Nitrite Using the System Nitrite Redutase/Nafion/Methyl Viologen - A Voltammetric Study”, Biosens. Bioelectron. 22(11), 2485-2492.

H. Chen, C. Mousty, S. Cosnier, C. Silveira, J.J.G. Moura, M.G. Almeida (2007) “Highly Sensitive ccNiR Biosensor Electrical-Wired by [ZnCrAQS2] for Nitrite Determination”, Electrochem. Comm. 9, 2240-2245.

C.M. Silveira, S.P. Gomes, A.N. Araújo, C.M.C.M. Couto, M.C.B.S.M. Montenegro, R. Silva, A.S. Viana, S. Todorovic, J.J.G. Moura, M.G. Almeida (2010) “An Efficient Mediatorless Biosensor for Nitrite Determination”, Biosens. Bioelectron., 25(9) 2026-2032.

C. Silveira, J. Baur, S. Cosnier, J.J.G. Moura, M. Holzinger, S. Cosnier, M.G. Almeida (2010) "Enhanced direct electron transfer of a multihemic nitrite reductase on SWCNT modified electrodes", Electroanalysis, 22 (24), 2973–2978.

M.G. Almeida, A.S. Serra, C. Silveira, J.J.G. Moura (2010) ”Nitrite Biosensing Via Selective Enzymes – A Long But Promising Route”, Sensors, 10(12) 11530-11555; doi:10.3390/s101211530.

A.S. Serra, S. Jorge, C. Silveira, J.J.G. Moura, E. Jubete, E. Ochoteco, M.G. Almeida (2011) ”Cooperative use of Cytochrome cd1 Nitrite Reductase and its Redox Partner Cytochrome c552 to Improve the Selectivity of Nitrite Biosensing” Anal. Chim. Acta, 693 (1-2), 41-46.