Negative peaks

What you expect

What you got

Disease Medicine
Recorder connections Check polarity
Refractive index of mobile phase higher than that of solute Change mobile phase
Vacancy peaks Originate from great difference in composition between sample solvent and mobile phase. Dissolve sample im mobile phase
Mobile phase more absortive than sample components Use mobile phase that is transparent at the wavelength used
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Disease	Medicine
Recorder connections	Check polarity
Refractive index of mobile phase higher than that of solute	Change mobile phase
Vacancy peaks	Originate from great difference in composition between sample solvent and mobile phase. Dissolve sample im mobile phase
Mobile phase more absortive than sample components	Use mobile phase that is transparent at the wavelength used

Send comments to:
Prof. Dr. H.J. Chaves das Neves
Department of Chemistry
Faculdade de Ciências e Tecnologia
Universidade Nova de Lisboa
2825 Monte da Caparica
Portugal

hcn@dq.fct.unl.pt