Hyphenation of HPLC to MS

Table of Contents

Hyphenation of HPLC to MS

The analytical problem in complex matrices

The classical approach

The specific detection approach

Intrinsically incompatible

Solved in HRGC- direct inlet

What About HPLC?

Why HPLC?

4 Main reasons

Intrinsically incompatibleas well

In HPLC we need to consider a broader range of operating parameters

Separation is function of K and peak broadening

HPLC side vs. MS side

May affect

Resolution

You have the compound

You get the fragments(and bad separations, noise, low performance, band broadening, remixing?)

And the reconstruction...

The interface

Interface means...

Most common interface devices

Thermospray

Thermospray

Electrospray

Heated nebulizer

The particle beam

Why a particle beam?

You get full spectra

Although not easy to operate

The particle beam

HRGC of a underivatized fracction from Arbutus unedo L. fruits

Same fraction after TMS

LC-PBMS

Structural information from PB-MS is decisive

Postulated structure from rationalization of EI-MS data

That's all folks

Email: hcn@dq.fct.unl.pt

Home Page: http://www.dq.fct.unl.pt/qof/intro.html

Other information:
©Professor H. J. Chaves das Neves, 1997

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